Quick Overview for FGF1 Protein (ABIN2721040)
Target
See all FGF1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human FGF acidic / FGF1 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is less than or equal to 0.1 ng/ml, in the presence of 10 ug/ml heparin, corresponding to a specific activity of > 1 x 10^7 units/mg.
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Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) (Active) protein (His tag)
FGF1 Origin: Mouse Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 1-155) (Active) protein (His tag,GST tag)FGF1 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (Active) proteinFGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 46-155) protein (GST tag)FGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 1-155) protein (GST tag)FGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) protein (His-IF2DI Tag)FGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) protein (His tag)FGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fibroblast Growth Factor 1 (Acidic) (FGF1) proteinFGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
Fibroblast Growth Factor 1 (Acidic) (FGF1) proteinFGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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Alternative Name
- Fgf Acidic,fgf1
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Molecular Weight
- 15.9 kDa
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NCBI Accession
- NP_036978
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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