GSK3 alpha Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722263

This Recombinant GSK3 alpha protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for GSK3 alpha Protein (Myc-DYKDDDDK Tag) (ABIN2722263)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This GSK3 alpha protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human GSK3 alpha protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Dunning, McGauran, Willén, Gouras, OConnell, Linse: "Direct High Affinity Interaction between Aβ42 and GSK3α Stimulates Hyperphosphorylation of Tau. A New Molecular Link in Alzheimer's Disease?" in: ACS chemical neuroscience, Vol. 7, Issue 2, pp. 161-70, (2016) (PubMed).

Target Details

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: Gsk3 alpha

Background: This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Molecular Weight: 50.8 kDa

NCBI Accession: NP_063937

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect