HPD Protein (Transcript Variant 2) (His tag)

Catalog No. ABIN2723013

This Recombinant HPD protein is produced in Escherichia coli (E. coli).

Quick Overview for HPD Protein (Transcript Variant 2) (His tag) (ABIN2723013)

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This HPD protein is labelled with His tag.

Characteristics:

• Recombinant human HPD / PPD (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM PMSF, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Alternative Name: Hpd,ppd

Background: The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 47.1 kDa

NCBI Accession: NP_001165464