IGF1 Protein (Transcript Variant 1)
Quick Overview for IGF1 Protein (Transcript Variant 1) (ABIN2723525)
Target
See all IGF1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Insulin-like growth factor I / IGF1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 3-15 ng/ml, corresponding to a specific activity of 0.6-3.3 x 105 units/mg, determined by the dose dependent stimulation of MCF-7 cell proliferation.
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Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein
IGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) (AA 1-115) (Active) proteinIGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His-IF2DI Tag)IGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein (His tag)IGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-138) protein (GST tag)IGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 1 x PBS
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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Alternative Name
- Insulin-Like Growth Factor I,igf1
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Molecular Weight
- 7.8 kDa
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NCBI Accession
- NP_034642
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Pathways
- RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
Target
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