IL12B Protein
Quick Overview for IL12B Protein (ABIN2723546)
Target
See all IL12B ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin 12b (Il12b) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.10 to 0.20 ng/ml, corresponding to a specific activity of 1.0 - 0.5 x 10^7 units/mg, determined by the dose dependent stimulation of IFN&gamma production by mouse splenocytes.
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Interleukin 12 beta (IL12B) (AA 23-328) (Active) protein (His tag)
IL12B Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 12 beta (IL12B) protein (His tag)IL12B Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 12 beta (IL12B) (AA 229-328) protein (GST tag)IL12B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)IL12B Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)IL12B Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
Interleukin 12 beta (IL12B) (AA 23-328) protein (Fc Tag)IL12B Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Interleukin 12 beta (IL12B) (AA 23-328), (full length) protein (GST tag)IL12B Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Interleukin 12 beta (IL12B) (AA 23-327), (full length) protein (His tag)IL12B Origin: Sheep Host: Yeast Recombinant > 90 % ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL12B (Interleukin 12 beta (IL12B))
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Alternative Name
- Interleukin 12b (Il12b)
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Molecular Weight
- 57.5 kDa
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NCBI Accession
- NP_032378
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Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Target
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