IL12A Protein
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- Target See all IL12A Proteins
- IL12A (Interleukin 12 alpha (IL12A))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Interleukin-12 alpha / IL12A protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Top Product
- Discover our top product IL12A Protein
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Interleukin 12 alpha (IL12A) (AA 57-253) protein
IL12A Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Interleukin 12 alpha (IL12A) (AA 144-253) protein (GST tag)IL12A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 12 alpha (IL12A) (AA 23-219) protein (His tag)Crystallography grade IL12A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 12 alpha (IL12A) (AA 23-215) protein (His tag)Crystallography grade IL12A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IL12A (Interleukin 12 alpha (IL12A))
- Alternative Name
- Interleukin-12 Alpha,il12a (IL12A Products)
- Synonyms
- CLMF Protein, IL-12A Protein, NFSK Protein, NKSF1 Protein, P35 Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p35 Protein, Il-12 p35 Protein, IL12p40 Protein, IL12 Protein, IL-12 p35 Protein, interleukin 12A Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12A Protein, IL12B Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein
- Background
- This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
- Molecular Weight
- 57.2kD
- NCBI Accession
- NP_000873
- Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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