IL-15 Protein (Transcript Variant 3)
Quick Overview for IL-15 Protein (Transcript Variant 3) (ABIN2723622)
Target
See all IL-15 (IL15) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin-15 / IL15 (transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per ug protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.10-0.50 ng/ml, corresponding to a specific activity of 2-10 x 10^6 units/mg, determined by the dose dependent stimulation of CTLL-2 cells proliferation.
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Interleukin 15 (IL15) (AA 49-162) protein
IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)IL15 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 15 (IL15) (AA 49-162), (full length) protein (His tag)IL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) proteinIL15 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-15 (IL15) (Interleukin 15 (IL15))
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Alternative Name
- Interleukin-15,il15
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Background
- Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 12.8 kDa
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NCBI Accession
- NP_000576
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Pathways
- JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
Target
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