IL-7 Protein (Transcript Variant 1)

Catalog No. ABIN2723750

Recombinant IL-7 protein expressed in HEK-293 Cells.

Quick Overview for IL-7 Protein (Transcript Variant 1) (ABIN2723750)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: Transcript Variant 1

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Interleukin-7 / IL7 (transcript variant 1) protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.20 - 1.0 x 10^6 units/mg, determined by PHA-activated human PBL proliferation induced by human IL-7 in a dose dependent manner.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 1 x PBS

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Interleukin-7,il7

Background: The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010].

Molecular Weight: 18.8 kDa

NCBI Accession: NP_000871

Pathways: JAK-STAT Signaling