IL-7 Protein (His tag)

Catalog No. ABIN7320603

Product Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This IL-7 protein is labelled with His tag.

Purpose: Recombinant Mouse Interleukin-7/IL-7 Protein (His Tag)(Active)

Sequence: Glu26-Ile154

Characteristics: Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 25-160 pg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Interleukin-7/IL-7 (IL7 Products)

Synonyms: IL7 Protein, IL-7 Protein, A630026I06Rik Protein, Il-7 Protein, hlb368 Protein, interleukin 7 Protein, IL7 Protein, Il7 Protein

Background:

Background: Mouse interleukin-7(IL-7) is the member of hemopoietin family which is important to the differentiation, proliferation, and survival of lymphocyte. Mouse IL-7 shares approximately 88 % aa sequence identity with rat IL-7 and 58-60 % with human, equine, bovine, ovine, porcine, feline and canine IL-7. It is widely expressed in primary and secondary lymphoid tissues cell and stromal epithelial cells of the thymus, bone marrow, and intestines. IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. It is important for proliferation during certain stages of B-cell maturation. IL-7 contributes to the maintenance of all na?ve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2. It is required for optimal T cell-dendritic cell interaction.

Synonym: IL-7, IL-7 interleukin-7, interleukin-7, Lymphopoietin -1, PBGF

Molecular Weight: 15.9 kDa

UniProt: P10168

Pathways: JAK-STAT Signaling