MAPKAP Kinase 3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2725454

Recombinant MAPKAP Kinase 3 protein expressed in HEK-293 Cells.

Quick Overview for MAPKAP Kinase 3 Protein (Myc-DYKDDDDK Tag) (ABIN2725454)

Target: MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This MAPKAP Kinase 3 protein is labelled with Myc-DYKDDDDK Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human MAPKAP Kinase-3 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Biological Activity Comment: MAPKAPK3 activity verified in a biochemical assay:,MAPKAPK3 activity verified in a biochemical assay:

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))

Alternative Name: Mapkap Kinase-3

Background: This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein.

Molecular Weight: 42.8 kDa

NCBI Accession: NP_004626

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades