MLH1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2726101

This Recombinant MLH1 protein is expressed in HEK-293 Cells.

Quick Overview for MLH1 Protein (Myc-DYKDDDDK Tag) (ABIN2726101)

Target: MLH1 (MutL Homolog 1 (MLH1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This MLH1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human MLH1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: Mlh1

Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009].

Molecular Weight: 84.4 kDa

NCBI Accession: NP_000240

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response