NEDD4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
This Recombinant NEDD4 protein is expressed in HEK-293 Cells.
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Catalog No. ABIN2727061
$956.40
Plus shipping costs $50.00, if applicable $20.00 dry ice
20 μg
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United States
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Quick Overview for NEDD4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2727061)
Target
See all NEDD4 Proteins
NEDD4
(Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
Protein Type
Recombinant
Origin
Human
Source
HEK-293 Cells
Application
Antibody Production (AbP), Standard (STD)
Purity
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This NEDD4 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human NEDD4 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
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Alternative Name
- Nedd4
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Background
- This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.
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Molecular Weight
- 104 kDa
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NCBI Accession
- NP_006145
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Pathways
- Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2
Target
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