PAK4 Protein (Transcript Variant 1)
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- Target See all PAK4 Proteins
- PAK4 (P21-Activated Kinase 4 (PAK4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human PAK4 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 90 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Specific activity was determined as 5,595 pmoles/min/μg, according to the Zlyte assay protocol
- Top Product
- Discover our top product PAK4 Protein
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P21-Activated Kinase 4 (PAK4) (AA 1-591) (Active) protein (GST tag)
PAK4 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
P21-Activated Kinase 4 (PAK4) (AA 300-591) (Active) proteinPAK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
P21-Activated Kinase 4 (PAK4) (AA 1-591) (Active) protein (His-GST)PAK4 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
P21-Activated Kinase 4 (PAK4) (AA 1-259) protein (His tag)PAK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
P21-Activated Kinase 4 (PAK4) (AA 68-157) protein (GST tag)PAK4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (His tag)PAK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (His tag)Crystallography grade PAK4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
P21-Activated Kinase 4 (PAK4) (AA 1-593) protein (His tag)Crystallography grade PAK4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PAK4 (P21-Activated Kinase 4 (PAK4))
- Alternative Name
- Pak4 (PAK4 Products)
- Background
- PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- Molecular Weight
- 33.3 kDa
- NCBI Accession
- NP_005875
- Pathways
- RTK Signaling
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