Paxillin Protein (PXN) (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728328

Recombinant Paxillin protein expressed in HEK-293 Cells and has been mentioned in 2+ publications.

Quick Overview for Paxillin Protein (PXN) (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2728328)

Target: Paxillin (PXN)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This Paxillin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Paxillin / PXN (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Sharifi, Mowers, Drake, Collier, Chen, Zamora, Mui, Macleod: "Autophagy Promotes Focal Adhesion Disassembly and Cell Motility of Metastatic Tumor Cells through the Direct Interaction of Paxillin with LC3." in: Cell reports, Vol. 15, Issue 8, pp. 1660-72, (2016) (PubMed).

Wilson, Bradley, Shaw, Wagenmakers: "Paxillin and focal adhesion kinase colocalise in human skeletal muscle and its associated microvasculature." in: Histochemistry and cell biology, Vol. 142, Issue 3, pp. 245-56, (2014) (PubMed).

Target Details

Target: Paxillin (PXN)

Alternative Name: Paxillin,pxn

Background: This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).

Molecular Weight: 60.8 kDa

NCBI Accession: NP_002850

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling