PCK1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for PCK1 Protein (Myc-DYKDDDDK Tag) (ABIN2728410)
Target
See all PCK1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PCK1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PCK1 / PEPCK1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (His tag)
custom-made PCK1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (His tag)custom-made PCK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (GST tag)PCK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (His tag)PCK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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Alternative Name
- Pck1,pepck1
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Background
- This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
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Molecular Weight
- 69 kDa
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NCBI Accession
- NP_002582
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Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
Target
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