POLR2F Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729318

Recombinant POLR2F protein expressed in HEK-293 Cells.

Quick Overview for POLR2F Protein (Myc-DYKDDDDK Tag) (ABIN2729318)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This POLR2F protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human POLR2F protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: Polr2f

Background: This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants.

Molecular Weight: 14.3 kDa

NCBI Accession: NP_068809

Pathways: Regulatory RNA Pathways