PKC iota Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729628

This Recombinant PKC iota protein is produced in HEK-293 Cells.

Quick Overview for PKC iota Protein (Myc-DYKDDDDK Tag) (ABIN2729628)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This PKC iota protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human PRKCI protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: Prkci

Background: This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Molecular Weight: 68.1 kDa

NCBI Accession: NP_002731

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation