RAD51 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730410

Recombinant RAD51 protein expressed in HEK-293 Cells.

Quick Overview for RAD51 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730410)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This RAD51 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human RAD51 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: Rad51

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 36.8 kDa

NCBI Accession: NP_002866

Pathways: DNA Damage Repair