RAD52 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
Quick Overview for RAD52 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2730415)
Target
See all RAD52 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- This RAD52 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RAD52 (transcript variant 3) protein expressed in HEK293 cell.
- Produced with end-sequenced ORF clone
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RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 1-418) protein (His tag)
RAD52 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 60-282), (partial) protein (His-SUMO Tag)RAD52 Origin: Saccharomyces cerevisiae Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris-HCl, pH 7.3, 100 mM glycine, and 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
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Alternative Name
- Rad52
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Background
- The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
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Molecular Weight
- 46 kDa
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NCBI Accession
- NP_602296
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Pathways
- DNA Damage Repair
Target
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