RCHY1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for RCHY1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730656)
Target
See all RCHY1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This RCHY1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RCHY1 / PIRH2 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 1-261) protein (His tag)
custom-made RCHY1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 1-252) protein (GST tag)RCHY1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 1-261) protein (His tag)RCHY1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))
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Alternative Name
- Rchy1,pirh2
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Background
- The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
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Molecular Weight
- 29.9 kDa
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NCBI Accession
- NP_056251
Target
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