Ret Proto-Oncogene Protein (RET) (DYKDDDDK Tag)

Catalog No. ABIN2730741

This Recombinant Ret Proto-Oncogene protein is produced in Insect cells (Sf9).

Quick Overview for Ret Proto-Oncogene Protein (RET) (DYKDDDDK Tag) (ABIN2730741)

Target: Ret Proto-Oncogene (RET)

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Ret Proto-Oncogene protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human RET (C-term DDK tag, transcript variant 2) protein expressed in sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Ret Proto-Oncogene (RET)

Alternative Name: Ret

Background: This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

Molecular Weight: 35.3 kDa

NCBI Accession: NP_066124

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation