Ret Proto-Oncogene Protein (RET) (His tag)

Catalog No. ABIN7317437

This Recombinant Ret Proto-Oncogene protein is expressed in HEK-293 Cells.

Quick Overview for Ret Proto-Oncogene Protein (RET) (His tag) (ABIN7317437)

Target: Ret Proto-Oncogene (RET)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 92 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Ret Proto-Oncogene protein is labelled with His tag.

Purpose: Recombinant Human RET Kinase Protein (His Tag)

Sequence: Met 1-Arg 635

Characteristics: A DNA sequence encoding the extracellular domain of human RET (P07949-1) (Met 1-Arg 635) was fused with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Ret Proto-Oncogene (RET)

Alternative Name: RET Kinase

Background: CDHF12,CDHR16,HSCR1,MEN2A,MEN2B,MTC1,PTC,RET-ELE1,RET51,RET proto-oncogene, also known as RET, is a cell-surface molecule that transduce signals for cell growth and differentiation. It contains 1 cadherin domain and 1 protein kinase domain. RET proto-oncogene belongs to the protein kinase superfamily, tyr protein kinase family. RET proto-oncogene is involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates both cell death/survival balance and positional information. RET is required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, promotes the formation of Peyer's patch-like structures, modulates cell adhesion via its cleavage, involved in the development of the neural crest. RET proto-oncogene is active in the absence of ligand, triggering apoptosis. RET acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. It also regulates nociceptor survival and size, triggers the differentiation of rapidly adapting (RA) mechanoreceptors, mediated several diseases such as neuroendocrine cancers.

Molecular Weight:

Calculated MW: 69.1 kDa

Observed MW: 110-120 kDa

Gene ID: 5979

UniProt: P07949

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation