RFC3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for RFC3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730776)
Target
See all RFC3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This RFC3 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RFC3 / RFC38 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Replication Factor C (Activator 1) 3, 38kDa (RFC3) (AA 1-356) protein (His tag)
custom-made RFC3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Replication Factor C (Activator 1) 3, 38kDa (RFC3) (AA 1-356) protein (His tag)custom-made RFC3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Replication Factor C (Activator 1) 3, 38kDa (RFC3) (AA 1-356) protein (GST tag)RFC3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))
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Alternative Name
- Rfc3,rfc38
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Background
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
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Molecular Weight
- 40.4 kDa
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NCBI Accession
- NP_002906
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Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
Target
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