SMAD3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732221

This Recombinant SMAD3 protein is expressed in HEK-293 Cells.

Quick Overview for SMAD3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2732221)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This SMAD3 protein is labelled with Myc-DYKDDDDK Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human SMAD3 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Biological Activity Comment: SMAD3 Activity Verified in a DNA-binding Assay:,SMAD3 Activity Verified in a DNA-binding Assay:

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: Smad3

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Molecular Weight: 47.9 kDa

NCBI Accession: NP_005893

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy