SOX9 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732475

This Recombinant SOX9 protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for SOX9 Protein (Myc-DYKDDDDK Tag) (ABIN2732475)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This SOX9 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human SOX9 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Shi, Chiang, Labhart, Zhao, Yang, Mistretta, Henning, Maity, Mori-Akiyama: "Context-specific role of SOX9 in NF-Y mediated gene regulation in colorectal cancer cells." in: Nucleic acids research, Vol. 43, Issue 13, pp. 6257-69, (2015) (PubMed).

Target Details

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: Sox9

Background: The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Molecular Weight: 56 kDa

NCBI Accession: NP_000337

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development