SPARC Protein (Myc-DYKDDDDK Tag)
Quick Overview for SPARC Protein (Myc-DYKDDDDK Tag) (ABIN2732503)
Target
See all SPARC ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This SPARC protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human SPARC / Osteonectin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) (Active) protein (His tag)
SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) protein (His tag)SPARC Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 99-330) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His-IF2DI Tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Alternative Name
- Sparc,osteonectin
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Background
- This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 34.5 kDa
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NCBI Accession
- NP_003109
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Pathways
- Autophagy
Target
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