SPARC Protein (AA 18-303)
Quick Overview for SPARC Protein (AA 18-303) (ABIN7866060)
Target
See all SPARC ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 18-303
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Purpose
- Human SPARC Protein Recombinant
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Sequence
- APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
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Characteristics
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Protein Size: Recombinant SPARC migrates as an approximately 31 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human SPARC mature chain was expressed in E. Coli. Recombinant Human SPARC encodes amino acid residues 18-303.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) (Active) protein (His tag)
SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) protein (His tag)SPARC Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 99-330) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His-IF2DI Tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- SPARC was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Alternative Name
- SPARC
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Molecular Weight
- 31 kDa
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Pathways
- Autophagy
Target
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