TARDBP Protein (DYKDDDDK Tag)
Quick Overview for TARDBP Protein (DYKDDDDK Tag) (ABIN2733232)
Target
See all TARDBP ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This TARDBP protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human TAR DNA binding protein (TARDBP), full length, with C-terminal DDK tag,expressed in sf9 cells (full length, C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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TAR DNA Binding Protein (TARDBP) protein (Myc-DYKDDDDK Tag)
TARDBP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
TAR DNA Binding Protein (TARDBP) (AA 1-414) protein (His tag)TARDBP Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
TAR DNA Binding Protein (TARDBP) (AA 8-192) protein (His-IF2DI Tag)TARDBP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TAR DNA Binding Protein (TARDBP) (AA 1-260) protein (His tag)TARDBP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TAR DNA Binding Protein (TARDBP) (AA 1-414) proteinTARDBP Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
TAR DNA Binding Protein (TARDBP) (AA 1-414) protein (GST tag)TARDBP Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
TAR DNA Binding Protein (TARDBP) (AA 1-260) protein (GST tag)TARDBP Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 20 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- TARDBP (TAR DNA Binding Protein (TARDBP))
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Background
- HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
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Molecular Weight
- 45 kDa
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NCBI Accession
- NP_007366
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Pathways
- Positive Regulation of Peptide Hormone Secretion
Target
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