TK1 Protein (DYKDDDDK Tag)

Catalog No. ABIN2733610

This Recombinant TK1 protein is expressed in HEK-293 Cells.

Quick Overview for TK1 Protein (DYKDDDDK Tag) (ABIN2733610)

Target: TK1 (Thymidine Kinase 1, Soluble (TK1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This TK1 protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human Thymidine kinase 1 (TK1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TK1 (Thymidine Kinase 1, Soluble (TK1))

Alternative Name: Thymidine Kinase 1 (Tk1)

Background: The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.

Molecular Weight: 27 kDa

NCBI Accession: NP_003249