RYK Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734730

Recombinant RYK protein expressed in HEK-293 Cells.

Quick Overview for RYK Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2734730)

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This RYK protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Tyrosine-protein kinase RYK (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Alternative Name: Tyrosine-Protein Kinase Ryk

Background: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 67.9 kDa

NCBI Accession: NP_001005861

Pathways: RTK Signaling, WNT Signaling, Regulation of Cell Size