VAV1 Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for VAV1 Protein (Myc-DYKDDDDK Tag) (ABIN2735197)
Target
See all VAV1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This VAV1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human VAV1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Vav 1 Oncogene (VAV1) (AA 1-845) protein (His tag)
custom-made VAV1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Vav 1 Oncogene (VAV1) (AA 189-565) protein (His tag)VAV1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Vav 1 Oncogene (VAV1) (AA 189-565) protein (His tag)VAV1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vav 1 Oncogene (VAV1) (AA 681-790) protein (GST tag)VAV1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Phospholipase D2 (PLD2) is a guanine nucleotide exchange factor (GEF) for the GTPase Rac2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 49, pp. 19617-22, (2011) (PubMed).
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- VAV1 (Vav 1 Oncogene (VAV1))
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Alternative Name
- Vav1
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Background
- This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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Molecular Weight
- 98.1 kDa
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NCBI Accession
- NP_005419
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling
Target
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