VEGFA Protein (Transcript Variant 2)
Quick Overview for VEGFA Protein (Transcript Variant 2) (ABIN2735215)
Target
See all VEGFA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human VEGF-A (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.
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Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (Fc Tag)VEGFA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag,AVI tag,Biotin)VEGFA Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) (Active) protein (His tag,AVI tag,Biotin)VEGFA Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA Origin: Rat Host: Yeast Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)VEGFA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA Origin: Mouse Host: Yeast (Pichia pastoris) Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) protein (GST tag)VEGFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) protein (His tag)VEGFA Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Alternative Name
- Vegf-A
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Molecular Weight
- 14.1 kDa
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NCBI Accession
- NP_033531
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Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target
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