VEGFA Protein (Transcript Variant 2)

Catalog No. ABIN2735215

Product Details

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 2

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human VEGF-A (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin Level: Less than 0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: Vegf-A (VEGFA Products)

Synonyms: MVCD1 Protein, VEGF Protein, VPF Protein, Vegf Protein, Vegf120 Protein, Vegf164 Protein, Vegf188 Protein, Vpf Protein, vegf Protein, vegfa Protein, wu:fj82c06 Protein, VEGF-A Protein, VEGF164 Protein, eVEGF120 Protein, eVEGF164 Protein, vegf-a Protein, vpf Protein, vefg Protein, si:dkey-14d8.3 Protein, wu:fd42e02 Protein, vascular endothelial growth factor A Protein, vascular endothelial growth factor Aa Protein, vascular endothelial growth factor A L homeolog Protein, vascular endothelial growth factor Ab Protein, VEGFA Protein, Vegfa Protein, vegfaa Protein, vegfa.L Protein, vegfa Protein, vegfab Protein

Molecular Weight: 14.1 kDa

NCBI Accession: NP_033531

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling