FNDC5 Protein (AA 29-149, AA 32-152) (Fc Tag)

Catalog No. ABIN2745683

Recombinant FNDC5 protein expressed in HEK-293 Cells.

Quick Overview for FNDC5 Protein (AA 29-149, AA 32-152) (Fc Tag) (ABIN2745683)

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Protein Type: Recombinant

Origin: Human, Mouse, Rat

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 29-149, AA 32-152

Purification tag / Conjugate: This FNDC5 protein is labelled with Fc Tag.

Purpose: FNDC5:Fc (human) (rec.)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: FNDC5 (extracellular domain) (human, aa 32-152 / mouse, aa 29-149) is fused at the C-terminus to the Fc portion of human IgG1. FNDC5 (extracellular domain) has 100 % identity between mouse, rat and human.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Alternative Name: FNDC5

Background:

Fibronectin Type III Domain-containing Protein 5, Fibronectin Type III Repeat-containing Protein 2.

Irisin is a recently described exercise and PGC1-alpha-induced myokine secreted by skeletal muscle in mice and humans. Irisin has been shown to induce browning of white adipose tissue, but this role has been questioned and a new function in the control of mass and strength of cortical bone has been reported. The membrane fibronectin type III domain containing protein 5 (FNDC5) can be cleaved by an unknown protease to release the Irisin protein (aa 32-143 for human) and potentially also the FNDC5 (extracellular domain, aa 32-153 for human).

Molecular Weight: ~52kDa

UniProt: Q8NAU1

Pathways: Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation