NMNAT3 Protein (AA 1-252, full length, N-Term) (His tag)

Catalog No. ABIN2745774

Recombinant NMNAT3 protein expressed in Escherichia coli (E. coli).

Quick Overview for NMNAT3 Protein (AA 1-252, full length, N-Term) (His tag) (ABIN2745774)

Target: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-252, N-Term, full length

Purification tag / Conjugate: This NMNAT3 protein is labelled with His tag.

Purpose: NMNAT3 (human) (rec.) (His) (highly active)

Characteristics: Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.

Biological Activity Comment: >2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NAD+ per min.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NAD+ per min.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: 1 mg/mL

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 8.0, containing 300 mM sodium chloride, 1 mM DTT and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Alternative Name: NMNAT3

Background:

Alternate Names/Synonyms: Nicotinamide Mononucleotide Adenylyltransferase 3, NMN Adenylyltransferase 3, NaMN Adenylyltransferase 3, EC 2.7.7.18, EC 2.7.7.1

Product Description: Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.

Molecular Weight: ~27kDa (monomer)

UniProt: Q96T66