FLT1 Protein (Soluble)

Catalog No. ABIN2746017

This Recombinant FLT1 protein is produced in Insect cells (Sf9).

Quick Overview for FLT1 Protein (Soluble) (ABIN2746017)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect cells (Sf9)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: Soluble

Purpose: VEGFR-1, Soluble (human) (rec.)

Cross-Reactivity: Human

Characteristics: Human sVEGFR-1 (661aa).

Biological Activity Comment: The activity of sVEGFR-1 was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Soluble in water and most aqueous buffers. Reconstitute with PBS to a concentration not lower than 100 ng/mL.

Handling Advice: Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: After reconstitution, store at -80°C. Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: VEGFR-1

Background:

Vascular Endothelial Growth Factor Receptor 1, FLT-1, Fms-like Tyrosine Kinase 1

Recombinant human soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) is the naturally occurring form and is a glycosylated monomeric protein. The biological function of sVEGFR-1 seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor. VEGFR-1 is a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis and cancer cell invasion. It may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. It can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). It has a very high affinity for VEGFA and relatively low protein kinase activity. It may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of phospholipase C-gamma (PLCG) leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1 and may also phosphorylate CBL.

Molecular Weight: ~96kDa (monomer)

UniProt: P17948-2

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals