FLT1 Protein (AA 27-756) (His tag,AVI tag,Biotin)

Catalog No. ABIN5955009

Product Details

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 27-756

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FLT1 protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 27-756

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: VEGF R1 (FLT1 Products)

Synonyms: FLT Protein, FLT-1 Protein, VEGFR-1 Protein, VEGFR1 Protein, FLT1 Protein, flt1b Protein, sFlt1 Protein, AI323757 Protein, Flt-1 Protein, fms related tyrosine kinase 1 Protein, FMS-related tyrosine kinase 1 Protein, vascular endothelial growth factor receptor 1 Protein, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Protein, FMS-like tyrosine kinase 1 Protein, vascular endothelial growth factor receptor-1 Protein, FLT1 Protein, Flt1 Protein, CpipJ_CPIJ012087 Protein, flt1 Protein, FLT-1 Protein

Background: Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Molecular Weight: 85.9 kDa

NCBI Accession: NP_002010

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals