MGAT2 Protein (AA 30-447) (His tag)

Catalog No. ABIN2746041

Recombinant MGAT2 protein expressed in Human Cells.

Quick Overview for MGAT2 Protein (AA 30-447) (His tag) (ABIN2746041)

Target: MGAT2 (Mannosyl (Alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT2))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 30-447

Purification tag / Conjugate: This MGAT2 protein is labelled with His tag.

Purpose: Recombinant Human Mannoside Acetylglucosaminyltransferase 2/GlcNAc-TII (C-6His)

Sequence: RQRKNEALAP PLLDAEPARG AGGRGGDHPS VAVGIRRVSN VSAASLVPAV PQPEADNLTL RYRSLVYQLN FDQTLRNVDK AGTWAPRELV LVVQVHNRPE YLRLLLDSLR KAQGIDNVLV IFSHDFWSTE INQLIAGVNF CPVLQVFFPF SIQLYPNEFP GSDPRDCPRD LPKNAALKLG CINAEYPDSF GHYREAKFSQ TKHHWWWKLH FVWERVKILR DYAGLILFLE EDHYLAPDFY HVFKKMWKLK QQECPECDVL SLGTYSASRS FYGMADKVDV KTWKSTEHNM GLALTRNAYQ KLIECTDTFC TYDDYNWDWT LQYLTVSCLP KFWKVLVPQI PRIFHAGDCG MHHKKTCRPS TQSAQIESLL NNNKQYMFPE TLTISEKFTV VAISPPRKNG GWGDIRDHEL CKSYRRLQLD HHHHHH

Characteristics: Recombinant Human Mannoside Acetylglucosaminyltransferase 2/MGAT2 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Arg30-Gln447) of Human MGAT2 fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl, pH 8.0 .

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: MGAT2 (Mannosyl (Alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT2))

Alternative Name: GlcNAc-T-II

Sub Type: Fusionprotein

Background: Mannoside Acetylglucosaminyltransferase 2 (MGAT2) is a single-pass type II membrane protein that contains the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain and a C-terminal catalytic domain. MGAT2 catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Defects in MGAT2 are the cause of congenital disorder of glycosylation type 2A.
Alternative Names: Alpha-1,6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase, Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, NT-II, Mannoside Acetylglucosaminyltransferase 2, N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase II,

Molecular Weight: 49.3 kDa

UniProt: Q10469