MGAT2 Protein (AA 30-447) (His tag)

Catalog No. ABIN5853617

Product Details

Target: MGAT2 (Mannosyl (Alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT2))

Protein Type: Recombinant

Protein Characteristics: AA 30-447

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MGAT2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MRQRKNEALA PPLLDAEPAR GAGGRGGDHP SVAVGIRRVS NVSAASLVPA VPQPEADNLT LRYRSLVYQL NFDQTLRNVD KAGTWAPREL VLVVQVHNRP EYLRLLLDSL RKAQGIDNVL VIFSHDFWST EINQLIAGVN FCPVLQVFFP FSIQLYPNEF PGSDPRDCPR DLPKNAALKL GCINAEYPDS FGHYREAKFS QTKHHWWWKL HFVWERVKIL RDYAGLILFL EEDHYLAPDF YHVFKKMWKL KQQECPECDV LSLGTYSASR SFYGMADKVD VKTWKSTEHN MGLALTRNAY QKLIECTDTF CTYDDYNWDW TLQYLTVSCL PKFWKVLVPQ IPRIFHAGDC GMHHKKTCRP STQSAQIESL LNNNKQYMFP ETLTISEKFT VVAISPPRKN GGWGDIRDHE LCKSYRRLQ

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: MGAT2 (Mannosyl (Alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT2))

Alternative Name: MGAT2 (MGAT2 Products)

Synonyms: CDG2A Protein, CDGS2 Protein, GLCNACTII Protein, GNT-II Protein, GNT2 Protein, CG7921 Protein, Dmel\\CG7921 Protein, GlcNAc-TII Protein, GlcNAcT2 Protein, dMGAT2 Protein, MGC89475 Protein, Gnt2 Protein, AA407964 Protein, CH73-301C19.1 Protein, zgc:162268 Protein, mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase Protein, CG7921 gene product from transcript CG7921-RB Protein, mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase L homeolog Protein, mannoside acetylglucosaminyltransferase 2 Protein, MGAT2 Protein, Mgat2 Protein, mgat2.L Protein, mgat2 Protein

Background: MGAT2 is a golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Transcript variants with a spliced 5' uTR may exist, but their biological validity has not been determined. Recombinant human MGAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 50 kDa (439aa)

NCBI Accession: NP_002399

UniProt: Q10469