FcRn Protein (AA 22-297) (His tag,Strep Tag,AVI tag,Biotin)

Catalog No. ABIN2870570

This Recombinant FcRn protein is expressed in HEK-293 Cells.

Quick Overview for FcRn Protein (AA 22-297) (His tag,Strep Tag,AVI tag,Biotin) (ABIN2870570)

Target: FcRn (neonatal Fc Receptor (FcRn))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-297

Purification tag / Conjugate: This FcRn protein is labelled with His tag,Strep Tag,AVI tag,Biotin.

Brand: MABSol® - PrecisionAvi

Sequence: AA 22-297

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: The subunit FCGRT carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Twin Strep tag at the C-terminus. The protein has a calculated MW of 34.7 kDa (FCGRT), 14.7 kDa (B2M) . The protein migrates as 45-50 kDa (FCGRT), 15 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: FcRn (neonatal Fc Receptor (FcRn))

Alternative Name: FcRn

Background: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Molecular Weight: 34.7 kDa (FCGRT), 14.7 kDa (B2M)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process