APOC3 Protein (His tag)
Quick Overview for APOC3 Protein (His tag) (ABIN3008370)
Target
See all APOC3 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This APOC3 protein is labelled with His tag.
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Purpose
- Mouse Apolipoprotein C3 (APOC3) Protein
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Sequence
- EEVEGSLLLG SVQGYMEQAS KTVQDALSSV QESDIAVVAR GWMDNHFRFL KGYWSKFTDK FTGFWDSNPE DQPTPAIES
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Biological Activity Comment
- Not tested
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Apolipoprotein C-III (APOC3) (AA 21-99) protein (GST tag)
APOC3 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Apolipoprotein C-III (APOC3) (AA 21-99) protein (His tag)APOC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Apolipoprotein C-III (APOC3) (AA 1-99) protein (GST tag)APOC3 Origin: Human Host: Wheat germ Recombinant AbP, ELISA, PAr, WB
Apolipoprotein C-III (APOC3) protein (Myc-DYKDDDDK Tag)APOC3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- APOC3 (Apolipoprotein C-III (APOC3))
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Alternative Name
- Apolipoprotein C3
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Molecular Weight
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Calculated MW: 10.4 kDa
Observed MW: 21 kDa (determined by SDS-PAGE) Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the -
UniProt
- P33622
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Pathways
- Carbohydrate Homeostasis, Lipid Metabolism
Target
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