Endothelin 1 Protein (EDN1) (His tag)
Quick Overview for Endothelin 1 Protein (EDN1) (His tag) (ABIN3009250)
Target
See all Endothelin 1 (EDN1) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Endothelin 1 protein is labelled with His tag.
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Purpose
- Mouse Endothelin-1 (EDN1) Protein
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Sequence
- ENGVQSPP PSTPWRPRRS KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP NKATDQAVRC QCAHQKDKKC WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS FRVAKLKAEL YRDQKLTHNR AH
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Biological Activity Comment
- Not tested
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Endothelin 1 (EDN1) (AA 113-212) protein (GST tag)
EDN1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Endothelin 1 (EDN1) (AA 54-202) (Active) protein (His tag)EDN1 Origin: Rat Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Endothelin 1 (EDN1) (AA 53-90) protein (His-IF2DI Tag)EDN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Endothelin 1 (EDN1) (AA 1-212) protein (GST tag)EDN1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Endothelin 1 (EDN1) (AA 53-73) (Active) protein (GST tag)EDN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Endothelin 1 (EDN1)
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Alternative Name
- Endothelin 1
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Molecular Weight
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Calculated MW: 21.0 kDa
Observed MW (SDS-PAGE): 21 kDa, 23 kDa -
UniProt
- P22387
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Pathways
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
Target
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