Endothelin 1 Protein (EDN1) (AA 54-202) (His tag)
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- Target See all Endothelin 1 (EDN1) Proteins
- Endothelin 1 (EDN1)
- Biological Activity
- Active
- Protein Characteristics
- AA 54-202
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Origin
- Rat
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Endothelin 1 protein is labelled with His tag.
- Application
- Cell Culture (CC), Activity Assay (AcA)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- Endothelins (EDN) are small (21 amino acids) vasoactive peptides produced by many cell types including endothelial and epithelial cells, macrophages and fibroblasts. By binding to G-protein-linked transmembrane receptors, EDNs participate in vasoconstriction modulation and cell growth regulation. It has been proven that EDN1 has chemotaxis active on monocytes, thus chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of EDN1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106cells/mL in RPMI 1640 with 0.5%FBS) and EDN1 (20ng/mL, 40ng/mL and 80ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37°C with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows EDN1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of EDN1 occurs at 80ng/mL.
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Endothelin 1 (EDN1) (AA 113-212) protein (GST tag)
EDN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endothelin 1 (EDN1) (AA 53-90) protein (His-IF2DI Tag)EDN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Endothelin 1 (EDN1) (AA 1-212) protein (GST tag)EDN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endothelin 1 (EDN1) (AA 53-73) (Active) protein (GST tag)EDN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA Active
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- Application Notes
- Isoelectric Point: 9.7
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- Endothelin 1 (EDN1)
- Abstract
- EDN1 Products
- Synonyms
- et1 Protein, et-1 Protein, Edn1 Protein, EDN Protein, ET-1 Protein, ET1 Protein, PPET1 Protein, HDLCQ7 Protein, preproET Protein, Et1 Protein, fb14d01 Protein, wu:fb14d01 Protein, PPET-1 Protein, EDN1 Protein, edn1 Protein, edn1-a Protein, edn1-b Protein, endothelin 1 Protein, endothelin 1 S homeolog Protein, endothelin 1 L homeolog Protein, edn1 Protein, EDN1 Protein, Edn1 Protein, edn1.S Protein, edn1.L Protein
- Background
- Alternative Names: ET1, PPET1, Preproendothelin-1, Big endothelin-1
- Molecular Weight
- 19kDa
- UniProt
- P22388
- Pathways
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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