Gelsolin Protein (GSN) (His tag)

Catalog No. ABIN3009561

This Recombinant Gelsolin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Gelsolin Protein (GSN) (His tag) (ABIN3009561)

Target: Gelsolin (GSN)

Protein Type: Recombinant

Origin: Cow

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Purity: > 97 %

Product Details

Purification tag / Conjugate: This Gelsolin protein is labelled with His tag.

Purpose: Cow Gelsolin (GSN) Protein

Sequence: G NDIYQWCGSS SNRFERLKAT QVSKGIRDNE RSGRARVHVS EEGAEPEAML EVLGPKPALP AGTEDTAKED AANRKLAKLY KVSNGAGTMS VSLVADENPF AQGALRSEDC FILDHGKDGK IFVWKGRQAN TEERKAALKT ASDFISKMDY PRQTQVSVLP EGGETPLFKQ FFKNWRDPDQ TDGPGLSYLS SHIANVERVP FDAATLHTST AMAAQHGMDD DGRGQKQIWR IEGSDKVPVD PATYGQFYGG DSY

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose and Proclin-300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: Gelsolin (GSN)

Alternative Name: Gelsolin

Molecular Weight: Calculated MW: 31.6 kDa
Observed MW (SDS-PAGE): 35 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

Gene ID

5350077

UniProt: Q3SX14

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy