GREM1 Protein (His tag)
Quick Overview for GREM1 Protein (His tag) (ABIN3009707)
Target
See all GREM1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This GREM1 protein is labelled with His tag.
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Purpose
- Rat Gremlin 1 (GREM1) Protein
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Sequence
- KKKGSQ GAIPPPDKAQ HNDSEQTQSP PQPGSRTRGR GQGRGTAMPG EEVLESSQEA LHVTERKYLK RDWCKTQPLK QTIHEEGCNS RTIINRFCYG QCNSFYIPRH IRKEEGSFQS CSFCKPKKFT TMMVTLNCPE LQPPTKKKRV TRVKQCRCIS IDLD
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Biological Activity Comment
- Not tested
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Gremlin 1 (GREM1) (Active) protein (His tag)
GREM1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS Active
Gremlin 1 (GREM1) (AA 25-184) (Active) protein (Fc Tag)GREM1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Gremlin 1 (GREM1) (AA 25-184) protein (His-IF2DI Tag)GREM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Gremlin 1 (GREM1) (AA 25-124) protein (GST tag)GREM1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Gremlin 1 (GREM1) (AA 1-184) protein (GST tag)GREM1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Gremlin 1 (GREM1) (AA 25-184) (Active) protein (His tag)GREM1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose.
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Preservative
- Dithiothreitol (DTT), Other preservative
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- GREM1 (Gremlin 1 (GREM1))
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Alternative Name
- Gremlin 1
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Molecular Weight
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Calculated MW: 22.0 kDa
Observed MW (SDS-PAGE): 24 kDa -
Pathways
- Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location
Target
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