14-3-3 sigma/SFN Protein (His tag)
Quick Overview for 14-3-3 sigma/SFN Protein (His tag) (ABIN3011750)
Target
See all 14-3-3 sigma/SFN (SFN) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This 14-3-3 sigma/SFN protein is labelled with His tag.
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Purpose
- Mouse 14-3-3 Protein Sigma (SFN) Protein
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Endotoxin Level
- < 1.0 EU/μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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Stratifin (SFN) (AA 1-248) protein (His tag)
custom-made SFN Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stratifin (SFN) (AA 1-248) protein (His tag)custom-made SFN Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stratifin (SFN) (AA 1-248) protein (GST tag)SFN Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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Alternative Name
- Stratifin
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Molecular Weight
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Calculated MW: 31.5 kDa
Observed MW (SDS-PAGE): 33 kDa -
UniProt
- O70456
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Pathways
- p53 Signaling, Myometrial Relaxation and Contraction
Target
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