SUMO1 Protein (AA 1-101) (His tag,SUMO Tag)

Catalog No. ABIN7313674

This Recombinant SUMO1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SUMO1 Protein (AA 1-101) (His tag,SUMO Tag) (ABIN7313674)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-101

Purification tag / Conjugate: This SUMO1 protein is labelled with His tag,SUMO Tag.

Purpose: Recombinant Human SUMO1 Protein is produced by our E. coli expression system and the target gene encoding Met1-Val101 is expressed with a 6His tag at the N-terminus.

Sequence: MGSSHHHHHH SSGLVPRGSH MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNNTPKEL GMEEEDVIEV YQEQTGGHST V

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, pH 8.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1

Gene ID: 7341

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway