SUMO1 Protein (His tag)

Catalog No. ABIN6700550

Supplier Product No.: 009-001-t66

This Recombinant SUMO1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SUMO1 Protein (His tag) (ABIN6700550)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This SUMO1 protein is labelled with His tag.

Supplier Product No.: 009-001-t66

Supplier: Rockland

Purpose: SUMO1 (UBL1) recombinant protein-HIS Epitope

Purification: Recombinant human SUMO1 (UBL1) (2-97) was expressed in E. coli using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: SUMO1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. SUMO1 Protein is suitable for use in Western Blot. Expect a band approximately ~19 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: SUMO1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1

Background:

Synonyms: UBL1, DAP1, GMP1, SMT3, OFC10, SENP2, SMT3C, SMT3H3, PIC1, Small ubiquitin-related modifier 1

Background: SUMO1 (also known as UBL1) is a member of the SUMO (small ubiquitin-like modifier) protein family which functions in a manner similar to ubiquitin. SUMO1 is bound to target proteins as part of a post-translational modification system and it is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (1). SUMO1 may be involved in the repair of TOP1-mediated DNA damage (2). The sumoylation pathway plays a significant role in mammalian DNA damage response. SUMO1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Apoptosis/Autophagy, Cell Cycle, and NfkB Pathway research.

NCBI Accession: NM_003352

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway