CD276 Protein (CD276) (DYKDDDDK-His Tag)
Quick Overview for CD276 Protein (CD276) (DYKDDDDK-His Tag) (ABIN5505582)
Target
See all CD276 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CD276 protein is labelled with DYKDDDDK-His Tag.
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Characteristics
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- Recombinant human Purified recombinant protein of human CD276 Molecule (CD276), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 μg (C-term DDK/His tag, transcript variant 1) protein expressed in Human HEK293 cells.
- Produced with end-sequenced ORF clone
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Purification
- Purified
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CD276 (CD276) (AA 29-245) protein (His tag)
CD276 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD276 (CD276) (AA 27-461) protein (Fc Tag)CD276 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-245) protein (mFc-His Tag)CD276 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD276 (CD276) (AA 29-245) protein (His-Avi Tag,Biotin)CD276 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-248) protein (His tag)CD276 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-244) protein (Fc Tag)CD276 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD276 (CD276) (AA 28-140) protein (His-DYKDDDDK-Strep II Tag)CD276 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 237-461) protein (His-DYKDDDDK-Strep II Tag)CD276 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 28-238) protein (His-DYKDDDDK-Strep II Tag)CD276 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 29-245) protein (Fc Tag,AVI tag,Biotin)CD276 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 1 x PBS, pH 7.4, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CD276
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Alternative Name
- CD276 molecule
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Background
- The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 49 kDa
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NCBI Accession
- NP_001019907
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Pathways
- Cancer Immune Checkpoints
Target
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