Ret Proto-Oncogene Protein (RET) (DYKDDDDK-His Tag)

Catalog No. ABIN5505759

This Recombinant Ret Proto-Oncogene protein is produced in HEK-293 Cells.

Quick Overview for Ret Proto-Oncogene Protein (RET) (DYKDDDDK-His Tag) (ABIN5505759)

Target: Ret Proto-Oncogene (RET)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Ret Proto-Oncogene protein is labelled with DYKDDDDK-His Tag.

Characteristics:

• Recombinant human Purified recombinant protein of human ret proto-oncogene (RET), with C-terminal DDK/His tag, expressed in human cells, 20 μg (C-term DDK/His tag) protein expressed in Human 293HEK cells.
• Produced with end-sequenced ORF clone

Purification: Purified

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 1 x PBS, pH 7.4, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Ret Proto-Oncogene (RET)

Alternative Name: ret proto-oncogene

Background: This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

Molecular Weight: 70.5 kDa

NCBI Accession: NP_066124

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation